产品名称：HEK293细胞hOPA1基因敲除株

产品简称：HEK293 KO-hOPA1

产品货号：CGKO-M2390

修饰基因：hOPA1

基因ID：4976

修饰类型：敲除

转导方法：蛋白电转

抗性基因：—

克隆类型：杂合子

细胞鉴定：PCR，测序

种属来源：人

组织来源：胚肾

疾病特征：/

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%P/S

配套培养基货号：TCH-G190

传代比例：1:4-1:8，每2-3天换液一次

传代周期：24-48 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

"Xie QW, et al. Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes. Proc. Natl. Acad. Sci. USA 93: 4891-4896, 1996. PubMed: 8643499

Da Costa LT, et al. Converting cancer genes into killer genes. Proc. Natl. Acad. Sci. USA 93: 4192-4196, 1996. PubMed: 8633039

Graham FL, et al. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-72, 1977. PubMed: 886304

Graham FL, et al. Defective transforming capacity of adenovirus type 5 host-range mutants. Virology 86: 10-21, 1978. PubMed: 664220

Harrison T, et al. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology 77: 319-329, 1977. PubMed: 841862

Bodary SC, McLean JW. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J. Biol. Chem. 265: 5938-5941, 1990. PubMed: 1690718

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383"

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