病毒细胞产品

  • Jurkat细胞ATM基因敲除株(CGKO-M2203)

产品货号:CGKO-M2203

产品价格:15187元

产品名称:Jurkat细胞ATM基因敲除株

产品简称Jurkat KO-hATM

产品货号CGKO-M2203

修饰基因:hATM

因ID472

修饰类型:敲除

转导方法:蛋白电转

抗性基因:

克隆类型:纯合子

细胞鉴定:PCR,测序

种属来源:人

组织来源:外周血

疾病特征:急性T淋巴细胞白血病

细胞形态:淋巴母细胞样

生长特性:悬浮生长

培养体系:RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

配套培养基货号:TCH-G225

传代比例:1:3-1:6,每2-3天换液一次

传代周期:24-48 h

培养条件:气相:95%空气+5%CO2,温度:37℃

冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测:细菌、真菌、支原体检测均为阴性

参考文献:

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Gillis S, Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J. Exp. Med. 152: 1709-1719, 1980. PubMed: 6778951

Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed: 9284127

Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663

Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842

Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984

Kung SH, Medveczky PG. Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J. Virol. 70: 1738-1744, 1996. PubMed: 8627695

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082"


收货当天如何处理细胞?

收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。

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友情链接: 国家实验细胞资源共享平台 ATCC Zhang Lab 海星生物商城 火箭加速器
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