病毒细胞产品

  • 人肝癌细胞(HepG2)
  • 人肝癌细胞(HepG2)

细胞名称:人肝癌细胞(STR鉴定正确)

细胞别称:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2

货号:TCH-C196

规格:1X10^6

价格:1400元(推荐T25瓶,复苏发货1-2周)

|   使用说明书

MX020A6_TCH-C196_人肝癌细胞(HepG2).pdf


该细胞来源于一名15岁的白人少年的肝癌组织。该细胞表达甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、转铁蛋白、α-1-抗凝乳蛋白酶、结合珠蛋白、铜蓝蛋白、纤溶酶原、补体C4、C3激活物、纤维蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、视黄醇结合蛋白;表达胰岛素受体和胰岛素样生长因子IGFⅡ的受体;该细胞具有3-羟基-3-甲酰辅酶A还原酶和肝甘油三酯脂肪酶的活性。目前尚未证明该细胞中有HBV基因组。

细胞名称:人肝癌细胞

细胞简称HepG2

产品货号TCH-C196

种属来源

组织来源

疾病特征肝癌

细胞形态上皮细胞样

生长特性贴壁生长

培养体系MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+1%P/S

配套培养基货号:TCH-G196

传代比例:1:2-1:4,每2-3天换液一次

传代周期72-96 h

培养条件气相:95%空气+5%CO2,温度:37℃

冻存条件60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测细菌、真菌、支原体检测均为阴性

参考文献:

"Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983

Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

Cuthbert C, et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed: 9169468

Deleersnyder V, et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401

Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004

Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Lewis W, et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed: 8622980

Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649

Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491

Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812

Lieber A, et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024

Dubuisson J, Rice CM. Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615"


收货当天如何处理细胞?

收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。

点击可查看下载《细胞收货处理方法说明》.jpg

友情链接: 国家实验细胞资源共享平台 ATCC Zhang Lab 海星生物商城
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