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TCH-C348-人结肠腺癌细胞（L-15空气培养）（SW480）培养要点.pdf

MX044A3_TCH-C348_人结肠腺癌细胞（SW480）.pdf

SW480源自一位51岁白人男性患者的原位直肠腺癌，而SW620源自同一病人一年后的淋巴结转移灶。该细胞CSAp和直肠抗原3阴性；角蛋白阳性；p53基因第273位密码子的G→A突变引起Arg→His替代，309位密码子的C→T突变导致Pro→Ser替代；细胞p53蛋白表达水平升高；癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性；未检测到癌基因N-myc的表达；不表达Matrilysin（一种与肿瘤侵袭相关的金属蛋白酶）。有报道称该细胞表达GM-CSF。ras原癌基

细胞名称：人结肠腺癌细胞

细胞简称：SW480

产品货号：TCH-C348

种属来源：人

组织来源：结肠

疾病特征：结肠腺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：L-15+10%FBS+1%P/S

配套培养基货号：TCH-G348

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：空气，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

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收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。