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MX030A4_TCM-C749_小鼠成纤维细胞（L-929）.pdf

1948年三月建立了NCTCclone929(小鼠结缔组织)，细胞系L的克隆。细胞系L是较早建立的连续培养细胞系之一，而clone929是较早的克隆株。从一只100日龄的雄性C3H/An小鼠的正常皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L。第95代的细胞系L使用毛细管法分离单细胞建立了clone929。检测发现鼠痘病毒阴性。

细胞名称：小鼠成纤维细胞

细胞简称：L-929

产品货号：TCM-C749

种属来源：小鼠

组织来源：皮下结缔组织

疾病特征：/

细胞形态：成纤维细胞样

生长特性：贴壁生长

培养体系：MEM+10%FBS+1%P/S

配套培养基货号：TCM-G749

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

"Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324

Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517

Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 393311

ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07.

ASTM International Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0895-84 (Reapproved 2006).

U.S. Pharmacopeia General Chapters:<87>Biological Reactivity Tests, in vitro. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed: 13233820

Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943."

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收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。