产品说明书:
MU129A4_ADMC-C106_C57BL6小鼠脂肪间充质干细胞电子版.pdf
细胞名称:HyCyte™ C57BL/6小鼠脂肪间充质干细胞
细胞简称:C57BL/6 ADSC
产品货号:ADMC-C106
种属来源:小鼠,C57BL/6品系
组织来源:脂肪
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养基货号:ADMC-G102
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
参考文献:
Zuk PA ,Zhu M ,Mizuno H ,et al. Multilineage cells from human adipose tissue:implications for cell-based therapies.Tissue Eng.2001;7(2):211-228.
Gronthos S, Franklin DM,Leddy HA, et al. Surface proteincharacterization of human adipose tissue-derivedstromal cells. J Ceilular Physiology.2001;189:54-63.
Cousin B, Andre M, Arnaud E, et al. Reconstitution of lethallyirradiated mice by cells isolated from adipose tissue.Biochemical Biophysical Res Communications. 2003,301:1016-1022.
Sen A,Leacurrie YR,Sujkowska D,et al.Adipogenic potentialof human adipose derived stromal cells from multiple donorsis heterogeneous.J Cell Biochem.2001;81:312-319.
Lee JA,Parrett BM,Conejero JA,et al.Biological alchemy-engineering bone and fat from fat-derived stemcells.Ann Plast Surg.2003;50(6):610-710.